Stem Cell Technology Certificate


Stem Cell Technology Certificate


 City College of San Francisco 


Students who complete the Stem Cell Certificate are eligible to apply for the Stem Cell Internship program.  Internships are payed, six-month positions in local companies or academic institutions.

The Stem Cell Technology certificate program is designed to prepare students for a research or clinical cell biology position working with mammalian cells and tissue culture. In the 21C course, specific techniques pertaining to the culture and maintenance of both embryonic and adult stem cells will be learned. Students should have a working knowledge of algebra from previous academic or work experience.


Program Coordinator:

Dr. Carin Zimmerman      

Tel: (415) 239-3857



Current schedule of classes

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 There are three entries to this program:


 FIRST ENTRY: For students with a college background in biology, chemistry and math. This certificate is designed to be completed in two semesters.


Required courses 

BTEC 21A       Mammalian Cell Culture (2 units) 6 weeks – 9 hrs/week

BTEC 21B       Fluorescent Cell Technology (2 units) 6 weeks – 9 hrs/week

BTEC 21C       Stem Cell Technology (3 units) 8 weeks – 9 hrs/week

BTEC 22         Immunoassay: ELISA (1 unit)

BTEC 23         Western Blotting Techniques (1 unit)

BTEC 24         Introduction to PCR (1 unit)

BTEC 25         Analytical PCR Technology (1 unit)


SECOND ENTRY: For students without a science/math college background. Students must acquire the necessary knowledge and skills in biology and chemistry prior to enrollment in Biotechnology/Stem Cell coursework.

For students with a strong math and science high school background. (3+ semesters)

First semester

BIO 11 + Lab Introduction to the Science of Living Organisms (4 units)

                       or BIO 101A (better)

CHEM 32       Introduction to Medical Chemistry (4 units) or Chem 208A (better)


Second semester

CHEM 40      Introduction to Chemical Principles (4 units) or CHEM 101A (better)

                        Pre-requisite: MATH 860 or higher or BTEC 108A

BTEC 22       Immunoassay: ELISA (1 unit)

BTEC 24       Introduction to PCR (1 unit)


Third+ semester:

BTEC 21A       Mammalian Cell Culture (6 weeks)

BTEC 21B       Fluorescent Cell Technology (6 weeks)

BTEC 21C       Stem Cell Technology (8 weeks)

BTEC 23         Western Blotting Techniques (1 unit)

BTEC 25         Analytical PCR Technology (1 unit)


THIRD ENTRY: For students with a limited science and math background.

The Bridge to Biotech Laboratory Assistant Program  should be completed before enrolling in the regular first semester (second entry) of science coursework. This program successfully prepares students for more rigorous college level coursework; it consists of three courses that form a learning community and must be taken together: BTEC 10, Research Skills for Career Opportunities, BTEC 108A, Practical Math; and BTEC 107 Language Skills for Tech. The Bridge Program is offered at the Mission and Southeast Campuses.  For more information, see the Bridge to Biotech Laboratory Assistant Program home page.





Stem Cell Internship Program

Six-month, paid internships in local stem cell research laboratories.  

3T3 cells stained with MitoTracker green

Mitochondria in mouse 3T3 cells stained with MitoTracker Green in BTEC 21B.

Tunnel assay

TUNEL assay detects apoptotic COS-7 cells (green staining).  From BTEC 21B.

Fluorescent activated cell analyzer

Fluorescent activated cell analyzer

Coulter counters

Coulter counters


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