Biolyzer Panel

SOP for Kodak IBI Biolyzer:

  1. PURPOSE: 

    To instruct individuals in the proper use of the Kodak Biolyzer Rapid Analysis instrument to obtain critical analyte results. 

  2. SCOPE: 

    This document will allow individuals to learn how to operate and calibrate the Kodak Biolyzer Rapid Analysis System to track vital analyte consumption/production of living organisms.


    It will be the responsibility of the user(s) of the instrument to properly operate, maintain and document the status of the equipment. 


    1. Kodatrol I and II: QC reagents used in instrument QC.
    2. Analyte: chemical/substance being measured.
    3. Quality Control: test the quality of the reagents used in the test.
    4. Calibration: tests the instruments performance.

    1. Kodak Biolyzer Rapid Analysis System
    2. Kodatrol I and II
    3. Cal Plus
    4. Kodak Analyte Slide(s)
    5. Kodak Biolyzer pipet tips
    6. Charge cord for pipet

    1. Instrument Preparation:

      1. Turn on the instrument. Allow the instrument to warm up for 15-20 minutes prior to using.
      2. Plug in the charge cord to the pipettor. Switch the red button on top of the pipettor so the indicator hole is showing. Ensure the LCD on the pipettor reads [CH].
      3.  Remove the control and calibration reagents(if needed) and test samples from the freezer and/or refrigerator. Allow them to reach room temperature before using (15-30 minutes).
    2. Maintenance:

      Perform daily maintenance prior to using the instrument by:

      1. Empty slide disposal box and check the paper level.
      2. Check pipettor tip for residual dirt. If cleaning is necessary refer to instrument manual for directions.
    3. Instrument Calibration:

      • Compare the analyte slide(s) lot number to the calibration log sheet to verify the instrument has been calibrated using the same lot numbers.
      • Calibration is necessary if the slide(s) lot number are different than the calibration log sheet and/or it is the first time the instrument is used in the semester. See attachment A for calibration protocol.
    4. Quality Control Testing:

      1. Enter the date in the Biolyzer by the following steps:

        1. Press the [SHIFT] then the [SERVICE] key.
        2. At the [OPTION] prompt, type 17 then ENTER.
        3. Enter date as MM-DD-YY, then ENTER. NOTE: This will return you to the OPTION prompt.
        4. Exit out of service mode by pressing the [SHIFT] then the [SERVICE] key.
      2. Remove slides to be QC'd (2 per analyte) from their foil package. NOTE: slides must be used within 15 minutes of opening package.
      3. Place the slide in the slide track, line up slide as shown on slide lever. Manually push the slide advance lever to move the slide into the spotting position.
      4. Enter ID of specimen (if necessary) by pressing [SAMPLE ID] key and enter sample name/number. If no ID is necessary the machine will automatically assign it a numerical number, ie. 1,2,3, etc....).
      5. Place pipet tip firmly on the pipettor. Ensure the pipettor LCD reads either [CH ASP] or [CH Lo #]. If the pipettor LCD does not read either of these, push the white button of the front of the pipettor. It should display the above codes. If not refer to manual for help.
      6. Place pipet tip in the test solution and depress the white button on the front of the pipettor.The pipettor should read [CH ASP] followed by [----], [----]. These dashes correspond to 2 seconds. IMPORTANT: Remove the pipet tip from the solution before it beeps,(2 second).
      7. Visually inspect the pipet tip to ensure the pipettor has aspirated 2 ul of air after pipettor beeps. If no air is present, the tip must be discarded and repeat step 6.4.6.
      8. Insert the pipet tip into the spotting locator ensuring pipet is properly seated. Dispense the sample by pushing the white button again. NOTE: the pipettor LCD should read CH DSP.
      9. Remove the pipettor from the locator and discard the pipet tip by pushing the grey button on the side of the pipettor. Repeat steps 6.4.1-6.4.9 for each analyte to be tested.
      10. Record QC results on the logsheet located next to the instrument. Verify QC is in range by comparing results to the QC information range sheet supplied in each of the Kodatrol kits. If QC is out of range, repeat testing and/or refer to troubleshooting section of the operating manual. QC must be in range prior to testing any test samples.

    1. Follow steps 6.4 for sample testing, except replace control reagents with test samples. NOTE: If QC was done prior to samples, it is not necessary to enter the date again, skip to section 6.4.2.
    2. Shut off instrument after biolyzer has completed testing and has printed results.

    1. Calibration logsheet
    2. QC logsheet

    Calibration procedure for the Kodak IBI Biolyzer Rapid Analysis System. 


    1. Handle all reagents using universal precautions. Wear protective eyeware, gloves and labcoat. Reagents are made using human blood products.
    2. Wash hands after handling biological agents and chemical reagents.



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