DNA Gel Box

SOP Owl Horizontal Agarose Gel Electrophoresis Apparatus

1. Prepare gel box for casting:
   Set tray in chamber with gasketed ends pressed against the walls of the buffer chamber and insert the comb selected.
2. Cast gel:
   Pour cooled gel solution (about 60oC) into tray.
3. Remove tray and rotate:
   Remove tray carefully from chamber and turn 90o so that the comb(s) is/are on the same side as the negative electrode (black).
4. Replace tray:
   Replace the tray into the chamber, exposing the open ends of the gel to the buffer chamber.
5. Add buffer:
   Pour enough running buffer to completely cover the gel; careful rocking of the tray will eliminate any air bubbles under the tray.
6. Remove comb(s):
   Carefully remove comb(s) by lifting up slowly. Do not tear or damage wells.
7. Load the samples:
   Follow specifications for well bolumes below; in most cases we will be using a 10 tooth comb.
8. Replace lid:
   Carefully slide lid onto the unit: the lid electrodes should connect to the chamber electrodes.
9. Connect to power supply and turn on.
   Follow protocol for the power supply being used.
10. Cleaning the system:
    Use tepid water and soft cloth to clean comb, tray, chamber and lid.
    DO NOT use soap or brush on any part of the gel box.
    Rinse in deionized water. 

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