Microcentrifuge

Microcentrifuge

SOP for Corning Costar Microcentrifuge

A. Preparation

  1. Be sure the rotor is properly seated on the motor shaft hub. The two notches on the underside of the rotor should engage the drive pins protruding from the hub.
  2. Secure the rotor by holding the outside while firmly tightening the knurled rotor nut.
  3. Connect the transformer to the centrifuge and plug into an electrical outlet.
  4. Press the centrifuge down firmly on a smooth surface so that the suction cup feet will lock the centrifuge in place.
  5. When removing the rotor, gently rock from side to side while lifting.
  6. Always let the rotor stop before opening the cover. The pilot light will go off when the rotor is stopped.
  7. In order to open cover, the solenoid unit must be plugged in. Do not force cover open.
  8. The operating temperature of the centrifuge and tubes should be between 4 and 56 degrees C.

B. Loading

  1. Always load an even number of microcentrifuge tubes.
  2. Always use the same size tube in opposite positions.
  3. Always balance the liquid in opposite tubes within 75 µl.
  4. Always be sure the tubes are seated all the way down in the rotor nest.
  5. Always close the caps on the tubes.

C. Controls

  1. Momentary Operation: Turn rotary switch to "pulse" and rotor will spin only while "start" button is pressed.
  2. Timed Operation: Set rotary switch to desired number of minutes and press "start" button. Unit will beep at the end of the timed run. If the cover is lifted during a run, the rotor will stop but the timer will continue to count. 
  3. Continuous Operation: Set rotary switch to "continuous" position and press "start" button. To stop, set switch back to "pulse" position.
  4. Speed Operation: Set rotary switch to required speed from 1000 to 10,000 rpm and press "start" button.

D. Safety

  1. Use only standard microcentrifuge tubes as described in the instruction manual. Make sure that tubes fit and are positioned properly.
  2. Avoid opening cover while rotor is spinning (pilot light is on). If cover is opened, rotor will brake to a complete stop within about 5 seconds. 
  3. Routinely inspect centrifuge and rotor for cleanliness and damage. Do not run with any signs of damage.
  4. Do not use cracked tubes or tubes whose filters or caps do not fit properly.
  5. Avoid prolonged exposure to direct sunlight or ultraviolet light. Keep lid closed when not in use.
  6. A new rotor should be installed annually.

E. Troubleshooting

  1. Scraping sound: Stop motor, open cover, and look for signs of a problem. Remove tubes and run again. If scraping persists, make certain rotor is seated properly with shaft pins in rotor notches.
  2. Excessive vibration: This is a sign of unbalance. Check rotor for position of tubes opposite each other, and check tubes for balanced weight.

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