12/23/08
Olivier Henry
Minneapolis, MN
Email: r1295@bio-link.org
Date Available:
Present
Location Desired:
Any
Objective:
A research/process development senior scientist position in organic, medicinal or bioorganic chemistry within a pharmaceutical or biotechnology company
Education
Post Doctorate Biochemistry
Post Doctorate Organic Chemistry
PhD Organic Chemistry
Master Biochemistry
Biotechnology Skills
Postdoctoral experience in Organic Chemistry and Biochemistry.
Organic Synthesis: multistep synthesis (total synthesis), free radical chemistry, tetracyclines chemistry, multistep synthesis of radioactive (32P) compounds, solid phase peptide synthesis, HPLC.
Biochemistry: Bacterial cell growth, protein purification (FPLC, immunoaffinity chromatography) and analysis (western blot), enzymology (kinetic analysis), photoaffinity labelling, rubber biosynthesis.
Spectroscopic Techniques: NMR spectra 1D and 2D on Brüker and Varian Spectrometers, high resolution mass spectrometry (CI, ESI), FT-IR, Fluorescence, UV.
Computer skills: InsightII (introduction to molecular modelling), Unix, Perl, Bibliography Databases (SciFinder, Beilstein Commander), ChemDraw, Chemwindow, KaleidaGraph, MS Office, EndNote.
Team work and supervision abilities.
English (fluent), French (mother tongue).
Work Experience
2004-Present University of Minnesota Minneapolis, MN
Performed computer modelling (InsightII) to design bisubstrate inhibitors of PGGTase.
Purified mammalian enzymes expressed in E. coli.
Synthesized bisubstrate inhibitors via solid phase synthesis.
Characterized inhibitors by enzyme kinetic methods.
Synthesized photoactive analogues of FPP (enzyme substrate) in radiolabelled form (32P).
Performed photolabelling and western blot experiments to identify binding site for FPP for several prenyltransferases.
Worked in teams, supervised graduate and undergraduate students.
2002-2004 University of Rochester Rochester, NY
Synthesized tetracycline derivatives, a collaboration with Innapharma, Park Ridge, NJ. Synthesized AHR receptor ligand analogues, a collaboration with Medical Center, University of Rochester , Rochester, NY.
Found the mechanism of an unusual decarboxylation. Independent project.
1997-2002 Université des Sciences et Technologies du Languedoc Montpellier, France
Synthesized three metabolites of F2-Isoprostane (prostaglandin derivatives) involved in oxidative stress.
Investigated and applied free radical cyclization to the synthesis of the metabolites.
Investigated oxidative stress and its connection with isoprostanes.
Supervised master and undergraduate students.
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