02/04/09
Mahadev Kalyankar
Philadelphia, PA
Email: r1312@bio-link.org
Date Available:
Immediately
Location Desired:
Pennsylvania, New Jersy, Maryland, New York
Objective:
To obtain a Scientific Position in Biotech/Pharmaceutical Company where I can put forward my academic knowledge and skills.
Education:
Ph.D. Life Sciences
MS Biochemistry
BS Biology
Biotechnology Skills:
RESEARCH AND TECHNICAL SKILLS
Animal handling and study of in-vivo physiology: Insulin and glucose tolerance studies in small animal models, small surgical procedures to implant Alzet pumps with siRNA and anti-sense oligos for gene knock out studies. Isolate tissues & cells to perform various cell signaling studies, Glucose Tolerance Test and Insulin Tolerance Test, handling & performing basic procedures (e.g., blood collection, blood and tissue processing).
Biochemistry: Recombinant protein expression & purification, In-vitro protein phosphorylation, protein kinases & phosphatases assays, various cell based assays development, One and two-dimensional electrophoresis (2D gel both DIGE (Fluorescence Difference Gel Electrophoresis) and non-DIGE). Immunoprecipitation, blotting, RIA, ELISA.
Molecular Biology: cDNA cloning, vector construction, Northern and Southern blotting, DNA transfections, recombinant adenoviral vector construction & virus preparation, antisense approach for gene silencing methods using siRNA oligos, construction of siRNA expressing adenoviruses, & protein-protein interaction (FRET analysis).
Cell and Tissue Culture: Hands on experience in growing various kinds of cell lines, cancer cell lines, insulin sensitive cell lines, Endothelial cells, Smooth Muscle cells. Matrigel and soft agar assays to assess anchorage independent growth of transformed cells.
Cell Biology: Immunocytochemistry, fluorescence & confocal microscopy, Live cell Imaging.
Histology: Perfusion, Fixation, cryostat tissue sectioning, immunohistochemistry (IHC), immunofluorescence (IFA).
Computer Knowledge: NCBI GenBank search, Blast and related tools, MS Word, Excel, Power point, Photoshop, Sigma Plot, Vector NTI, NIH Image.
Work Experience:
2005 â€" Present Instructor of Medicine: Department of Medicine, Division Endocrinology, Diabetes and
Metabolic Diseases, Thomas Jefferson University, Philadelphia, PA.
 Study of insulin-sensitizing, anti-inflammatory, anti-oxidant and vascular protective
effects of adiponectin in-vitro and in-vivo. Cardio. Res. 2008, 78: 376-84, ATVB
2008, 28: 899-905. JCI 2007, 117: 1718-1726, Diabetes 2006, 55: 1840-46
 Study of insulin signaling pathways in Nox4-knock out mouse model to identify
novel targets to develop effective therapies for the treatment or prevention of type 2 diabetes mellitus.
 Mentoring and supervising research assistants, technicians, graduate students and
senior endocrinology fellowship-trainees in the laboratory.

2000 â€" 2005 Post-doctoral Research Fellow: Department of Medicine, Division Endocrinology, Diabetes and Metabolic Diseases, Thomas Jefferson University, Philadelphia, PA.
 Investigated molecular details of the signaling pathways used by insulin to generate
oxidant signal that enhances the tyrosine phosphorylation of proteins in the insulin
signaling pathway by inhibition of thiol-dependent protein tyrosine phosphatases
such as PTP1B. JBC 2001, 276: 21938-42; JBC 2001, 276: 48662-69; Cell
Signaling 2004, 16: 323-31.
 Investigated the role of NADPH Oxidase catalytic subunit homolog Nox4 in
insulin-receptor couple oxidant generation and insulin sensitizing effects. This novel
area of research in insulin signaling will have application in new approaches in
enhancing insulin action in Type II diabetes and clinical states of insulin resistance.
Mol Cell Biol. 2004, 24: 1844-54.
1999 - 2000 Post-Doctoral Research Fellow: Department of Medicine, Fels Institute for Cancer Res & Mol. Biol., Philadelphia, PA.
 Changes in the expression of microfilament-associated proteins, such as tropomyosins (TMs) are commonly found in malignantly transformed cells, and tropomyosin-1 (TM1) expression is consistently abolished in human breast carcinoma cell lines, suggesting that the loss of TM1 could be a common biochemical event in the transformation of mammary epithelium. By stable expression of tropomyosin-1 protein in human breast cancer epithelial cell line MCF-7, I demonstrated that TM1 functions as a suppressor of transformation. Exp Cell Res. 2002, 279: 40-51.
1994 - 1999 Doctoral Research Fellow: University of Hyderabad, Hyderabad, India.
 Using animal model of fetal alcohol syndrome, I investigated the effects of alcohol
on various signal transduction pathways, in particular Protein Kinase C, CaM Kinase II and Tyrosin Kinases in rat brain during pre- and postnatal ethanol exposure.
Archives of Biochem. & Biophys. 1998, 356: 249-57; Neurochemical Research. 1998, 23: 1179-1184.
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