03/20/09
Gayathri Gopalan
Banaglore, karnataka, india
Email: r1328@bio-link.org
Date Available:
06/01/2009
Location Desired:
Objective:
A passionate science researcher experienced at working effectively in teams and independently seeking a challenging career in Life science.
Education:
2001-2005: Ph.D. (Biological Sciences), National University of Singapore (NUS)
1998- 2000: M.Sc. (Plant Science), University of Madras, India (CGPA: 5.6 /6.0).
1995 -1998: B.Sc. (Botany), University of Madras, India (overall percentage: 85%).
Biotechnology Skills:
Molecular biology:
1 PCR.
2 Making competent cells with E.coli.
3 Transformation of Ecoli with plasmid.
4 Growth and purification of protein using E.coli system.
Protein Chemistry:
1 Protein production and purification.
2 Inclusion body production and purification.
3 Protein crystallization.
4 X-ray diffraction of protein crystals.
5 Chromatography: HPLC, Size Exclusion, Ion Exchange, Specific Affinity, Ni-NTA, TLC,GC
6 Gel electrophoresis: SDS PAGE, Agarose.
7 Absorbance and Fluorescent Spectroscopy.
8 Electro spray Ionization (ESI).
9 Dynamic Light Scattering.
10 Circular Dichrosim.
11 Isothermal Titration Calorimetry.
Structural Biology:
Data Collection
Rigaku RU-200 rotating anode X-ray generator. Experienced in collecting data in Synchrotron.
Data Processing
DENZO, HKL2000,MOSFLM, d*TREK.
Structure Determination and Refinement
CCP4, XPLOR / CNS, XTALVIEW, SOLVE, PHASES Graphical O, GRASP, PYMOL, MOLSCRIPT, RASTER 3D
COMPUTER LITERACY:
LANGUAGES: C, BASIC and HTML
OS PLATFORMS: Windows (3.x, 98, NT, 2000, XP), UNIX, MS-DOS
PACKAGES: MS Word, Excel, Powerpoint, Adobe Photoshop
Work Experience:
2006- present : Self-employed tutor.
2005-2006: Postdoctoral Research Fellow, Arizona State University (ASU), USA.
Supervisor: Dr. R. Watcher.
Research areas:
1. Structural and Biochemical characterization of fluorescent proteins. Investigations on Crystal structure of dsFP483, a cyan florescent protein. Characterization of chromophore formation and maturation by biochemical and spectroscopy methods.
2. Crystallization and structural Investigations of cerulean, an enhanced cyan florescent protein
2001-2005: Research Scholar, National University of Singapore (NUS), Singapore.
Supervisor: Associate Prof. K. Swaminathan.
Research areas:
1. Structure Determination of FKBP-Type Immunophilin from the Arabidopsis chloroplast. Initiated project to understand the redox regulation of plant immunophilins. Solved the first plant immunophilin structure, AtFKBP13.Biophysical characterization of the plant immunophilins both in oxidized and reduced state.
2. Structural Studies on L-aspartate decarboxylase (panD) from Mycobacterium tuberculosis. Initiated project to understand the lipid metabolism of the pathogen. Solved the structure of L-aspartate decarboxylase, an important protein in pathothenate pathway.The structure serves as model for structure based drug design for Tuberculosis.
3. Phospholipase A2 and its inhibitor protein from snake venom. Worked on PLA2 from Russell viper. Studied the effect of PLA2 inhibitor from python on snake venom.
RESPONSIBILITIES:
• Initiated, planned and carried out research works, interpreting data, presenting results and conclusions
• Presented research work in various national conferences and through international technical journals
• Submitted project proposals to various funding agencies
• Supervised research students
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