07/16/09
Paresh Chothe
Augusta, GA
Email: r1341@bio-link.org
Date Available:
Any
Location Desired:
USA
Objective:
Seeking a Challenging position in a Dug Discovery and Development Group and would like to work in team for the adavancement of drug discovery process and patient health care
Education:
PhD in Biochemistry and Molecular Biology M. Pharm B. Pharm
Biotechnology Skills:
Cell Biology:
Mammalian cell culture to study the function of various transporter proteins.
Expression of membrane transporters in mammalian cell lines as a potential target and drug delivery mechanism Transient / stable transfection (Lipofectamine/Electroporation) of recombinant plasmids in mammalian cells.
Isolation of enterocytes and colonocytes from mouse and rat intestine and colon respectively Immunofluorescence analysis Flow Cytometry
Molecular Biology:
Isolation and purification of DNA, total RNA, mRNA from tissues and mammalian cells.
RT-PCR, Real Time PCR, Northern blotting.
Biochemistry:
Protein determination
Protein extraction and western blotting
Uptake studies using radiolabeled substrates in mammalian cells.
Tissue uptake studies using mouse/rat intestine, colon and kidney Brush border membrane vesicle preparation mouse/rat intestine, colon and kidney Radio-iodination of opioid peptides and oligo-peptides
Xenous Laevis Oocyte Usage Skills:
Electrophysilogy Preparation of Poly(A) mRNA cRNA preparation Heterologous expression of membrane transporter protein in X. laevis oocyte Uptake studies to characterize transporter protein expressed in oocyte Electrophysiological studies to characterize transporter protein expressed in oocyte using two electrode voltage clamping technique
Analytical Research:
Spectroscopic and chromatographic method development
Work Experience:
08/2005â€"present
Graduate Research Assistant, Biochemistry and Molecular Biology Department at Medical College of Georgia, Augusta Georgia USA.
08/2003â€"07/2005
Teaching assistant, Pharmaceutical Chemistry, Bharati Vidyapeeth’s College of Pharmacy, CBD, Navi Mumbai, MS, India. Responsibilities of conducting examinations, test grading & development of laboratory experiments for undergraduate students.
08/2002â€"04/2003
Teaching assistant in Pharmaceutical Analysis, Government College of Pharmacy, Vidyanagar, Karad, MS, India. Responsibilities of conducting examinations, test grading & development of laboratory experiments for diploma and undergraduate students.
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