07/17/09
Rachana
Elk Grove Village, IL
Email: r1342@bio-link.org
Date Available:
With two weeks of notice soon
Location Desired:
Any potential place where there is opportunity for job to apply my education and experience and yet desire to learn more.
Objective:
Looking forward to apply and share my skills and techniques for research-based and exploratory job positions.
Education:
MS- Cell and Molecular Biology.
BS- Genetics (Gold Medalist)
Biotechnology Skills:
Molecular Cloning and Cell Culture techniques learned to apply in research of looking Bax gene to relate its’ function with apoptosis in cancerous cells with following details:
• Performed RNA isolation, followed by RT-PCR, and Gel validation. (Invitrogen Kit).
• Designed primers for “Bax gene”, followed by RT-PCR and PCR to amplify the selected gene using another Invitrogen kit; PCR purification by spin-column technology implementing QIA quick PCR purification kit.
• Enzymatic digestion using Eco-RI and KpnI to ligate Bax gene into PC DNA 3.1 vector, followed by Gel Validation.
• Heat shock transformation of plasmid construct into DH5α cells and conducted Mini Prep assay after bacterial cultures; verification of colonies for transformation with EcoRI and KpnI digestion and Gel validation.
• Accountable for aseptic techniques of Mammalian cell culture of PC 3 cell lines, sub-culturing, splitting, harvesting, and freezing; transfection of PC 3 cells with Bax gene and actin control with EX Gen 500 reagent.
• Western blotting analysis for protein expression purified by SDS-PAGE, protein assay for concentration using Bio-rad kit.
• Visualization of immuno fluoresced HeLa cells using immunostaining technique.
• Familiar with flow cytometry.
Genetics Lab
• Cell division studies; calculation of seed exercise segregation ratio and goodness of fit for mono- and dihybrid crosses; chromosomal mutation analysis using UV rays; Barr bodies demonstration; induction of polyploidy; Chi-square analysis; biostatistics tests; immunoassays; DNA-RNA-protein purification and estimation; isolation of auxotroph by replica plating method; PCR; tissue culture; electrophoresis; etc.
Biochem Lab
• Amino acids separation study by chromatography; enzyme assay; quantitative estimation of nutrients; vitamin C & Iron; identification of bio-compounds; and qualitative analysis for protein; amino acid; fat; fatty acid; and cholesterol; titrametric and colorimetric analysis.
Additional Skills:
• GDP, GCP, good time management skills, good team-spirit, good inter-personal skills, good communication skills; basic computer skills, punctual, honest, friendly, optimistic, enthusiastic personality.
• Acknowledged with OSHA regulations and biohazard safety issues.
Work Experience:
Medstar Labs Inc. (Professional) 3/2007-Present
LAB TECHNICIAN
• Accountable for performing different laboratory tests using automated and sophisticated instruments such as:
GENPROBE-SB100 (PCR) and Immuno-assays for HIV& H. Pylori (ELISA).
CLINITEK (Urinalysis), bacterial cell culture, and urine microscopy.
DXC600 (Chemistry) and ACCESS 2 (Thyroid).
ACL 1000/ ELITE PRO (PT) (Coagulation), , LH750 (Hematology).
Lead Analyzer, blood bank, and sickle cell anemia detection and sedrate.
• Responsible for laboratory maintenance; preparing solutions; ordering supplies; and preparing inventory. Performs calibrations and troubleshooting as needed.
• Records results and perform data entry in automated computer systems. (Familiarity with Hex)
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