08/27/09
Kelley Bryan-McNeal
Clarksville, MD
Email: r1347@bio-link.org
Date Available:
8/31/09
Location Desired:
Maryland
Objective:
Research Associate I/II
Education:
Masters of Science in Agriculture, (2008) Kansas State University Department of Animal Sciences
Bachelors of Science in Agriculture, Kansas State University, Department of Animal Sciences
Biotechnology Skills:
- In vitro cell culture
- Recombinant DNA
- Electrofusion/Electroporation
- Bradford Assays
- Immunocytochemistry
- Quantitative RT-PCR
- DNA and RNA isolation
- Transfection
- Electrophoresis
- Gel Extraction
- Primer Design
- Confocal Microscopy
- Small Animal Handling
Work Experience:
• University of Maryland, College Park: 2009 - USDA grant: successfully transfected bovine fetal fibroblasts and human-NTERA2 cells with linearized plasmid DNA containing the Nanog promoter linked to reporter gene GFP. - Relevance of experiment is to further examine epigenetic regulation of induced pluripotent stem cells or application for somatic cell nuclear transfer
• Kansas State University Graduate Research Assistant: 2007-2008 - Created an in vitro extracellular matrix termed Pormatrix from the Wharton’s jelly of the pig umbilical cord (patent pending) - Cultured porcine umbilical cord matrix stem cells on Pormatrix and Matrigel to compare expression levels of three genes responsible for pluripotency. - Assisted in an in vivo experiment in which pig umbilical cord matrix stem cells were fed to neonate piglets and intestine was viewed via confocal microscopy to observe stem cell trafficking within the gut
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