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See Also: Equipment List
- With gloved hands, place all necessary materials in cabinet prior to use. (Consult batch culture checklist or SOP).
- Place bio-waste receptacle adjacent to cabinet.
- Spray and wipe down work surface with 70% IPA.
- Close glass door and turn on UV light for 15 minutes. This is located on top control panel.
- Once 15 minutes have elapsed, turn off UV light.
- Turn on flourescent light and receptacle switch. This is located on top control panel.
- Open glass door to 10 inch mark only or alarm will sound.
- Immediately turn on blower.
- Light bunsen burner according to SOP. This is located on the side of the bio-safety cabinet.
- Replace gloves prior to entering bio-safety cabinet.
- Spray all spinner flasks and media bottles with 70% IPA and wipe dry. Place inside of cabinet.
- Perform protocol while working in center of cabinet. Do not block intake grill. This ensures proper airflow.
- Once protocol is complete, turn off bunsen burner according to SOP.
- Wipe down working surface with 70% IPA.
- Turn off blower, fluorescent light and receptacle switch.
- Close glass door.
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