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CO2 Regulator for REVCO Incubator See Also: Equipment List  Start with all valves in the off position. Tank valve turned down clockwise completely. Regulator valve turned out counterclockwise until it feel free. Line valve turned down clockwise completely. Slowly open tank valve one full turn counterclockwise. Turn regulator valve clockwise until 15 psi is shown on gauge on left. Turn line valve counterclockwise until completely open. TOP Bio-Link.org Web: www.Bio-Link.org Email: info@Bio-Link.org NSF Award #9850325