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See Also: Equipment List
- Prepare gel box for casting:
Set tray in chamber with gasketed ends pressed against the walls of the buffer chamber and insert the comb selected. - Cast gel:
Pour cooled gel solution (about 60oC) into tray. - Remove tray and rotate:
Remove tray carefully from chamber and turn 90o so that the comb(s) is/are on the same side as the negative electrode (black). - Replace tray:
Replace the tray into the chamber, exposing the open ends of the gel to the buffer chamber. - Add buffer:
Pour enough running buffer to completely cover the gel; careful rocking of the tray will eliminate any air bubbles under the tray. - Remove comb(s):
Carefully remove comb(s) by lifting up slowly. Do not tear or damage wells. - Load the samples:
Follow specifications for well bolumes below; in most cases we will be using a 10 tooth comb. - Replace lid:
Carefully slide lid onto the unit: the lid electrodes should connect to the chamber electrodes. - Connect to power supply and turn on.
Follow protocol for the power supply being used. - Cleaning the system:
Use tepid water and soft cloth to clean comb, tray, chamber and lid.
DO NOT use soap or brush on any part of the gel box.
Rinse in deionized water.
| # Teeth in Comb | Well Volume |
|---|---|
| 8 | 65ul |
| 10 | 50ul |
| 12 | 40ul |
| 14 | 30ul |
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