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See Also: Equipment List
A. CLEANING, RINSING, EQUILIBRATING, STORING AND SHUTTING DOWN THE LC-100
- Cleaning the LC-100:
- If the system has been stored in 0.1 M NaOH, then proceed to part 2. If not, place all four buffer lines into 0.1 M NaOH.
- Ensure the outlet tubing from the injector is connected to the inlet of the detector. If not connect appropriate tubing in place.
- Turn on the instrument. Press "MENU".
- Select "FLUSH", enter.
- The LC-100 will flush the entire system (buffer lines, proportional valves, pumps and system tubing) with 0.1 M NaOH. When the system stops, repeat steps c-d. Once flow stops, leave the system sitting in caustic for 10 minutes.
- Rinsing the LC-100:
- Place all four buffer lines in sterile filtered deionized water and the waste line in a beaker.
- Turn on the instrument if not already on and press "MENU".
- Select "FLUSH", enter.
- Repeat steps b and c until the water exiting through the waste line tested with pH paper is £ 7.0. This ensures that all caustic has been removed from the system. The system is ready to be equilibrated.
- Equilibrating the LC-100 system for use:
- Place all four buffer lines in Equilibration buffer ( Tris-HCl, pH 6.0).
- Turn on the instrument. Ensure the outlet tubing from the detector is attached to the inlet tubing of the detector.
- Select "MENU".
- Select "FLUSH", enter.
- Repeat steps 2 &3.
- System is ready. See section C for protocol on Equilibrating the column
- Place all four buffer lines in 0.1 M NaOH. Ensure the outlet of the injector is hooked up to the inlet tubing of the detector.
- Press "MENU"
- Select "SHUTDOWN". This will flush caustic through the system at 10 ml/min for four minutes. Make sure you have enough caustic supply since no air should enter the system.
- After system stops, follow prompt for correct way of shutting down the instrument.
- Place a status tag on the instrument with the Name: LC-100
Status: Stored
USE by Date: one month from today
Step: 0.1 M NaOH
Date/ Initials:_______________ - Turn off the instrument and discard waste liquid appropriately.
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