DNA barcoding is a popular technique for undergraduate research projects. In this webinar, Dr. Linnea Fletcher describes how this technique can be adapted for use in biotechnology education programs by incorporating principles of working in a regulated environment such as GMPs (Good Manufacturing Practices).
The Austin Community College (ACC) Biotechnology program introduces students to the 5 P’s of GMP [people, premises, processes, products and procedures (paperwork)] while they're using DNA barcoding to identify samples. Combining GMPs with DNA barcoding makes learning cGMPs more interesting to ACC students.
DNA barcoding is a process where students isolate DNA, use PCR to amplify a selected region, and send their DNA samples to a company for sequencing. Once the DNA has been sequenced, the sample files can be uploaded to software systems like the DNALC's DNA Subway for further analysis. Sequence data can be exported from DNA Subway and submitted to the National Center for Biotechnology Information (NCBI).
Dr. Linnea Fletcher, InnovATEBIO, Austin Community College, Austin, TX